Normal genetic response to gamma irradiation in familial adenomatous polyposis

The present study, a co-operative project between three European institutes, was aimed at elucidating whether the APC gene in carriers of familial adenomatous polyposis coli (FAP) also causes some genetic sensitivity revealed by DNA damage and the yield of chromosome aberrations in peripheral blood lymphocytes exposed to gamma rays. In addition, it seemed of interest to study whether DNA repair is modified after irradiation of lymphocytes from FAP patients compared to controls. To this end, we have used the inhibition of the poly(ADP-ribose) polymerase (ADPRP) by 3-aminobenzamide (3ABA) and studied the effect of 3ABA on the frequency of DNA strand breaks and chromosome aberrations. The data indicate that FAP is not associated with an increased chromosomal sensitivity towards ionising radiation.