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Conserved sequence patterns in phages Mu and lambda DNA

B Stern1, A Molven, D Kamp

  • 1Laboratory of Biotechnology, University of Bergen, HIB, Norway.

Biochimica Et Biophysica Acta
|October 17, 1995
PubMed
Summary
This summary is machine-generated.

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Bacteriophages Mu and lambda share a conserved nucleotide pattern, not similar gene sequences, suggesting a common evolutionary origin. This finding offers a novel method for tracing distant phage relationships.

Area of Science:

  • * Molecular Biology
  • * Evolutionary Biology
  • * Bioinformatics

Background:

  • * Genetic maps of bacteriophages Mu and lambda can be aligned by gene function.
  • * Previous studies have not investigated sequence-level congruence between these phages.

Purpose of the Study:

  • * To determine if gene order congruence between bacteriophages Mu and lambda is reflected at the nucleotide sequence level.
  • * To investigate the basis of sequence similarity between bacteriophages Mu and lambda.

Main Methods:

  • * Sliding window analysis of nucleotide sequences from early gene regions of Mu and lambda.
  • * Comparison of sequence similarity scores between different phage regions and control sequences.
  • * Application of degenerated scoring matrices to identify contributing nucleotides.

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Main Results:

  • * Substantial nucleotide sequence similarity was found between the early regions of Mu and lambda.
  • * High similarity scores were also observed in cross-comparisons (Mu early vs. lambda late) and self-comparisons.
  • * Adenine and thymine residues, with specific clustering, were identified as the primary contributors to the conserved pattern.
  • * This pattern was not observed in control sequences from other phages, E. coli, or eukaryotic viruses.

Conclusions:

  • * The observed nucleotide sequence similarity between Mu and lambda is due to a common underlying pattern, not functional gene similarity.
  • * The data strongly support the hypothesis that bacteriophages Mu and lambda diverged from a common ancestral phage module.
  • * The developed approach provides a sensitive method for detecting distant evolutionary relationships among phages.