In-vitro models of B-lineage commitment

The development of mature B lymphocytes from multipotent progenitors follows a pathway of differentiation marked by a progressive restriction in lineage options. The requirements for progression through the B lineage developmental pathway have been investigated intensively and a number of critical components of the differentiation process have been identified. However, the genetic basis for lineage determination remains unresolved. Recently, a number of in-vitro assays have been established which support the development of committed B cell progenitors from multipotent cells. These assays have provided a novel system in which the process of B lineage commitment can be followed and manipulated. In this review we present a model of B-lineage progression from multipotent progenitors to committed B-cell progenitors and discuss potential mediators of the commitment process.