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Rolling in P-selectin-deficient mice is reduced but not eliminated in the dorsal skin
S Yamada1, T N Mayadas, F Yuan
1Steele Laboratory, Department of Radiation Oncology, Massachusetts General Hospital, Boston 02114, USA.
Abstract:
P-selectin-mediated rolling is believed to be important in the recruitment of leukocytes to tissue after ischemia-reperfusion injury. The dorsal skin chamber was used to examine differences in the rolling and stable adhesion of circulating leukocytes in subcutaneous (SC) vessels of P-selectin-deficient and age-matched wild-type mice, both under basal conditions and after ischemia-reperfusion. Rolling in the postcapillary venules in SC tissue of P-selectin-deficient mice was significantly lower than that in wild-type mice under the basal conditions and post-ischemia-reperfusion (P < .05), but was not eliminated by the deletion of the P-selectin gene. No significant difference between P-selectin-deficient and wild-type mice in shear rate or leukocyte-endothelial adhesion was observed up to 24 hours after ischemia-reperfusion. These results show that P-selectin-mediated rolling is not a prerequisite for ischemia-reperfusion-induced leukocyte-endothelial adhesion in the skin.
Insights
P-selectin plays a role in leukocyte rolling but is not essential for their adhesion following ischemia-reperfusion injury. This study found reduced rolling in P-selectin deficient mice, but adhesion remained similar to wild-type mice.
Area of Science:
- Immunology
- Vascular Biology
- Physiology
Background:
- Leukocyte recruitment to tissues is crucial following ischemia-reperfusion injury.
- P-selectin is implicated in the initial rolling of leukocytes, a key step in their adhesion to the vessel wall.
Purpose of the Study:
- To investigate the role of P-selectin in leukocyte rolling and adhesion in the skin after ischemia-reperfusion injury.
- To determine if P-selectin-mediated rolling is a prerequisite for leukocyte adhesion in this context.
Main Methods:
- Utilized the dorsal skin chamber model in P-selectin-deficient and wild-type mice.
- Examined leukocyte rolling and stable adhesion in subcutaneous vessels under basal and post-ischemia-reperfusion conditions.
Main Results:
- Leukocyte rolling was significantly lower in P-selectin-deficient mice compared to wild-type mice, both basally and post-injury (P < .05).
- However, leukocyte rolling was not completely abolished in P-selectin-deficient mice.
- No significant differences in leukocyte-endothelial adhesion were observed between the two groups up to 24 hours after ischemia-reperfusion.
Conclusions:
- P-selectin-mediated rolling is not essential for ischemia-reperfusion-induced leukocyte adhesion in the skin.
- Leukocyte adhesion following ischemia-reperfusion can occur independently of P-selectin-mediated rolling.