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Cloning and chromosomal localization of a pseudogene corresponding to a mRNA for a soluble IL-6 receptor
L Snyers1, J C Renauld, C Szpirer
1Institut Pasteur du Brabant, Bruxelles, Belgium.
DNA Sequence : the Journal of DNA Sequencing and Mapping
|January 1, 1995
Abstract:
A polymerase chain reaction assay (Lust J.A. et al. (1992). Cytokine 4:96-100) was used to detect a mRNA coding for a soluble IL-6 receptor in human hepatoma cells. In addition to the expected amplification product, we found a sequence (SR4) which could be aligned to it with 78% identity. After cloning and sequencing a genomic 2.5-2.7 kB EcoRI fragment containing SR4, this sequence turned out to be part of a pseudogene corresponding to the transmembrane domain deleted soluble IL-6 receptor. Screening of a panel of interspecies hybrids revealed that it maps to chromosome 9.