Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Engineering membrane proteins

J L Popot1, M Saraste

  • 1Institut de Biologie Physico-Chimique, Paris, France.

Current Opinion in Biotechnology
|August 1, 1995
PubMed
Summary
This summary is machine-generated.

Membrane protein engineering tailors protein structures for enhanced study and function. Techniques modify loops and helices, utilizing cysteine insertions and chemical synthesis for novel insights.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Early prognosticators of later TSPO-PET-measurable microglial activation in multiple sclerosis.

Multiple sclerosis and related disorders·2023
Same author

Humoral response to John Cunningham virus during pregnancy in multiple sclerosis.

Multiple sclerosis and related disorders·2018
Same author

Increased thyroid autoimmunity among women with multiple sclerosis in the postpartum setting.

Multiple sclerosis (Houndmills, Basingstoke, England)·2013
Same author

Cardiac positron emission tomography/computed tomography imaging accurately detects anatomically and functionally significant coronary artery disease.

Circulation·2010
Same author

Functional properties of the acetylcholine receptor protein.

Neurochemistry international·2010
Same author

Immunoregulatory factors in multiple sclerosis patients during and after pregnancy: relevance of natural killer cells.

Clinical and experimental immunology·2007
Same journal

Microbial C1 assimilation pathways for chemical synthesis: from native metabolism to synthetic design.

Current opinion in biotechnology·2026
Same journal

Medicinal plants fermentation: current knowledge and perspectives.

Current opinion in biotechnology·2026
Same journal

Fermented foods: lessons learned from metagenomics.

Current opinion in biotechnology·2026
Same journal

Microfluidic platforms for the transient transfection of mammalian cells: recent developments and challenges.

Current opinion in biotechnology·2026
Same journal

Harvesting insights from recent advances in yeast genomics for predictable and precision wine fermentation.

Current opinion in biotechnology·2026
Same journal

Minimal enzyme cascades for the aromatic-to-aromatic upgrading of lignin monomers.

Current opinion in biotechnology·2026
See all related articles

Area of Science:

  • Biochemistry
  • Structural Biology
  • Protein Engineering

Background:

  • Integral membrane protein research often parallels soluble protein studies.
  • Membrane proteins present unique engineering opportunities due to their distribution across lipidic and aqueous phases.

Purpose of the Study:

  • To explore specialized techniques in membrane protein engineering.
  • To highlight how these methods facilitate protein study and functional understanding.

Main Methods:

  • Engineering of extramembrane loops (shortening, elongation, insertion of functional sites).
  • Modification of transmembrane alpha-helices (deletion, duplication, replacement).
  • Cysteine residue insertion for structural analysis and reporter molecule attachment.
  • Chemical synthesis of protein fragments and whole proteins.

Related Experiment Videos

Main Results:

  • Engineering strategies enable easier purification, biochemical/biophysical studies, and crystallogenesis.
  • Modified helices aid in understanding membrane integration, assembly, and function.
  • Cysteine insertions allow detailed structural investigation and functionalization.
  • Chemical synthesis opens new avenues for studying small folding domains.

Conclusions:

  • Membrane protein engineering is a rapidly advancing field with a unique set of questions and techniques.
  • These engineering approaches provide powerful tools for dissecting membrane protein structure and function.