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Updated: Aug 18, 2026

Analyzing Large Protein Complexes by Structural Mass Spectrometry
Published on: June 19, 2010
Chemical techniques employed for the primary structural analysis of proteins and peptides
A S Inglis1, G E Reid, R J Simpson
1Joint Protein Structure Laboratory, Ludwig Institute for Cancer Research (Melbourne Branch) Parkville, Victoria Australia.
Abstract:
This chapter summarises modern microchemical approaches to the purification, identification and primary structure analysis of peptides and proteins. Discussion of high-sensitivity purification methods is restricted to two-dimensional polyacrylamide gel electrophoresis (2-DE) and microbore/capillary column reversed-phase high-performance liquid chromatography (RP-HPLC). Associated techniques are discussed, particularly with respect to analysis of the products with current automated amino acid sequencers and mass spectrometers.
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