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Composite structure of auxin response elements

T Ulmasov1, Z B Liu, G Hagen

  • 1Department of Biochemistry, University of Missouri, Columbia 65211, USA.

The Plant Cell
|October 1, 1995
PubMed
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Soybean GH3 gene promoters contain auxin response elements (AuxREs) with a TGTCTC core. These AuxREs require upstream sequences for full auxin inducibility, revealing a composite structure essential for gene regulation.

Area of Science:

  • Plant Molecular Biology
  • Gene Regulation
  • Plant Hormone Signaling

Background:

  • The soybean GH3 gene promoter is crucial for auxin-inducible gene expression.
  • Auxin response elements (AuxREs) are key cis-acting regulatory sequences mediating this response.
  • Previous studies identified the TGTCTC motif as a core component of AuxREs.

Purpose of the Study:

  • To investigate the functional role of the TGTCTC motif and adjacent sequences within AuxREs.
  • To elucidate the composite nature of AuxREs and their contribution to auxin inducibility.
  • To identify the DNA-binding proteins interacting with AuxRE components.

Main Methods:

  • Transient expression assays in carrot protoplasts.
  • Analysis of promoter activity in transgenic tobacco seedlings.

Related Experiment Videos

  • Site-directed mutagenesis of AuxRE sequences.
  • Electrophoretic mobility shift assays (EMSAs) with plant nuclear proteins.
  • Characterization of protein-DNA interactions using recombinant transcription factors.

    • Main Results:

    • The TGTCTC motif alone is necessary but insufficient for auxin inducibility.
    • Upstream sequences adjacent to TGTCTC are essential for conferring auxin inducibility and exhibit constitutive activity.
    • A composite AuxRE, comprising a constitutive element and the TGTCTC motif, was identified.
    • Specific plant nuclear proteins, including a G-box binding factor, interact with these constitutive elements.
    • A novel AuxRE engineered with a GAL4 binding site adjacent to TGTCTC demonstrated auxin-inducible expression.

    Conclusions:

    • Auxin response elements (AuxREs) possess a composite structure.
    • AuxREs consist of a constitutive element and a TGTCTC motif that confers auxin inducibility.
    • This composite structure is critical for strong auxin-inducible gene expression in plants.