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[Elaboration of the polymerase chain reaction system for subtype specific differentiation of influenza A virus

K V Tarasov, S Iu Tsvirkunova, T A Bekhtereva

Vestnik Rossiiskoi Akademii Meditsinskikh Nauk

|January 1, 1995

Abstract:

New subtype specific primers for hemagglutinin (H1, H2, H3) of influenza A viruses were selected. The specificity of the primers' performance was tested in a model experiment which used cDNA synthesized on the vRNA from the collection of influenza A viruses (H1, H2, H3 subtypes) as templates. The sensibility of the PCR system was determined on DNA plasmids- and RNA templates (with preliminary reverse transcription) and amounted to 10(2) and 10(5)-10(6) virion particles, respectively.

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