Measurement of phosphotyrosine phosphatase activity using the Folin-Ciocalteu phenol reaction
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1Institute of Medical Chemistry, University of Vienna, Austria.
Biochemistry and Molecular Biology International
|August 1, 1995
Abstract:
An assay for the estimation of phosphotyrosine phosphatase using the Folin-Ciocalteu phenol reaction to monitor enzyme activity is presented. The method is based on the property of the substrate phosphotyrosine not to react as a phenol until it is dephosphorylated. The method is sensitive, there is no interference from the use of detergents and it does not rely on special laboratory equipment to distinguish tyrosine from phosphotyrosine.