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Related Experiment Videos

Cell cycle-dependent morphological changes in the actin cytoskeleton induced by agents which elevate cyclic AMP

C J McNamee1, S R Pennington, P Sheterline

  • 1Department of Human Anatomy and Cell Biology, University of Liverpool, UK.

Cell Biology International
|September 1, 1995
PubMed
Summary

Agents increasing cyclic adenosine-5'-monophosphate levels cause Swiss 3T3 fibroblasts to develop arborised morphology by reorganizing actin filaments. This response is cell cycle-dependent, primarily occurring in the G1 phase.

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Area of Science:

  • Cell Biology
  • Biochemistry
  • Cytoskeleton Dynamics

Background:

  • Cyclic adenosine-5'-monophosphate (cAMP) is a crucial second messenger involved in various cellular processes.
  • Fibroblast morphology and actin cytoskeleton organization are critical for cell function and response to stimuli.

Purpose of the Study:

  • To investigate the morphological and cytoskeletal changes in Swiss 3T3 fibroblasts induced by agents that increase intracellular cAMP.
  • To determine the cell cycle dependency of the cAMP-mediated arborisation response.

Main Methods:

  • Treatment of sub-confluent Swiss 3T3 fibroblasts with agents that elevate intracellular cAMP.
  • Analysis of actin filament organization using fluorescence microscopy.
  • Assessment of cell cycle phase distribution and response timing after serum readdition.

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Main Results:

  • Agents increasing cAMP induced a highly arborised morphology in a proportion of fibroblasts.
  • Actin filaments reorganized from stress fibers to a network within arborisations without changes in overall polymerization.
  • The arborisation response was restricted to approximately 30% of cells in asynchronous cultures and occurred primarily in the G1 phase of the cell cycle.

Conclusions:

  • Increased intracellular cAMP triggers significant actin cytoskeleton reorganization leading to fibroblast arborisation.
  • The arborisation response is tightly regulated by the cell cycle, specifically occurring during the G1 phase.
  • These findings highlight the role of cAMP signaling in modulating cell shape and cytoskeletal dynamics in a cell cycle-dependent manner.