Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Developmental expression pattern screen for genes predicted in the C. elegans genome sequencing project

A S Lynch1, D Briggs, I A Hope

  • 1Department of Pure and Applied Biology, University of Leeds, UK.

Nature Genetics
|November 1, 1995
PubMed
Summary

This study uses genome sequencing data to analyze gene expression patterns in Caenorhabditis elegans. Researchers successfully identified developmental expression patterns for over half of the examined genes, aiding in functional genomics.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

BSHI/BTS guidance on crossmatching before deceased donor kidney transplantation.

International journal of immunogenetics·2021
Same author

The possibility of determining the age of colonies of clonally propagating herbaceous species from historic records: the case of Aster novi-belgii L. (first recorded as A. salignus Willd.) at Wicken Fen Nature Reserve, Cambridgeshire, England.

The New phytologist·2017
Same author

The effect of source of supplemental dietary calcium and magnesium in the peripartum period, and level of dietary magnesium postpartum, on mineral status, performance, and energy metabolites in multiparous Holstein cows.

Journal of dairy science·2017
Same author

ABO-incompatible renal transplantation without antibody removal using conventional immunosuppression alone.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2015
Same author

Transitional B lymphocytes are associated with protection from kidney allograft rejection: a prospective study.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2015
Same author

Meeting report: 3rd international transplant conference: how much risk can you take?

International journal of immunogenetics·2015

Area of Science:

  • Genomics
  • Developmental Biology
  • Molecular Biology

Background:

  • Genome sequencing projects generate vast amounts of data.
  • Understanding gene function requires analyzing their expression patterns.
  • Caenorhabditis elegans is a model organism for genetic studies.

Purpose of the Study:

  • To develop and apply a genome sequence-based expression pattern screen.
  • To analyze the expression of predicted genes from the Caenorhabditis elegans genome.
  • To make expression pattern information widely accessible.

Main Methods:

  • Utilized beta-galactosidase (lacZ) reporter gene fusions.
  • Constructed fusions using predicted Caenorhabditis elegans gene promoter regions.
  • Analyzed expression patterns in transformed C. elegans lines.

Related Experiment Videos

Main Results:

  • 67% of predicted genes in analyzed cosmids were amenable to the screening approach.
  • 54% of examined genes yielded a detectable developmental expression pattern.
  • Expression data is being compiled into computer databases.

Conclusions:

  • A genome sequence-based approach is effective for analyzing gene expression.
  • This method successfully identified developmental expression patterns for a significant portion of predicted genes.
  • The generated expression data will contribute to functional genomics in C. elegans.