Separation of juvenile hormone metabolites with a silica-based gel permeation column

Metabolites of juvenile hormone (JH) III, JH acid, JH diol and JH acid diol in an aqueous solution were separated by gel permeation chromatography using an UltraSpherogel SEC 2000 column. Each metabolite was eluted in an inclusion volume in the order JH acid diol, JH acid and JH diol. Although JH was the last compound eluted, it was co-eluted with a JH-binding protein (JHBP) when JHBP was present in the solution. Using this method, in vivo and in vitro JH catabolism studies were performed in the fifth stadium larvae of Bombyx mori.