Related Experiment Videos
Ochratoxin A in cereals and the BCR-M&T-projects
G M Wood1, A C Entwisle, S Patel
1Leatherhead Food Research Association, Surrey, U.K.
Natural Toxins
|January 1, 1995
Summary
This study improved ochratoxin A analysis in wheat by developing reference materials and comparing methods. Significant improvements in accuracy and reproducibility were observed in the second intercomparison study.
Area of Science:
- Food safety analysis
- Mycotoxin detection
- Analytical chemistry
Background:
- Ochratoxin A (OA) contamination in food poses a health risk.
- Accurate analytical methods and certified reference materials (CRMs) are crucial for OA regulation.
- Previous methods showed limitations in accuracy and reproducibility.
Purpose of the Study:
- To develop certified reference materials for ochratoxin A in wheat.
- To improve and harmonize analytical methodologies for OA determination.
- To assess the performance of different OA detection methods, including immunoaffinity columns.
Main Methods:
- Preparation of ochratoxin A reference materials in wheat matrix.
- Two intercomparison studies involving multiple European laboratories.
- Comparison of standard laboratory methods with novel immunoaffinity column techniques.
Main Results:
- The first intercomparison highlighted matrix interference issues, necessitating further method refinement.
- The second intercomparison demonstrated considerable improvements in OA determination.
- Immunoaffinity column methods showed promise compared to standard techniques.
Conclusions:
- The BCR project successfully developed reference materials for ochratoxin A in wheat.
- Harmonization of analytical methods and improved accuracy were achieved.
- The study advanced the reliable detection of ochratoxin A in food products.