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Reticulin-M, an antianaphylactic peptide secreted by reticulo-endothelial system
1Center of Physiological Recherch Univ. of Oradea.
Abstract:
I. Moldovan (1923) demonstrated in the blood ultrafiltrate from anaphylactically prepared guinea pigs, which believed to anaphylactic shock, or which were previously injected with coloidal substances as India ink to stimulate the RES, an antianaphylactic principle named Reticulin-M(R). Authors extracted R from organs rich in RES with acetone, established their, peptidic nature and tested it on anaphylactic prepared guinea pig uterine horns in vitro. The peptides were fractioned by high voltage paper electrophoresis. The fractions were cut up in four groups, eluted and tested. The second basic group was identified as active and concentrated by acetone, recipitation. In conclusion several techniques were used to obtain, to test, to isolate and to concentrate R, a natural antianaphylactic peptidic factor, which may be a cytokine.
Insights
Researchers isolated Reticulin-M (R), a natural antianaphylactic peptide factor, from RES-rich organs in guinea pigs. This peptide shows potential as a cytokine, offering new insights into anaphylaxis treatment.
Area of Science:
- Immunology
- Biochemistry
Background:
- Anaphylaxis is a severe allergic reaction.
- The Reticuloendothelial System (RES) plays a role in immune responses.
- An antianaphylactic principle, Reticulin-M (R), was previously identified in guinea pig blood.
Purpose of the Study:
- To extract, isolate, and characterize Reticulin-M (R).
- To confirm the peptidic nature and antianaphylactic activity of R.
- To investigate R's potential as a therapeutic agent for anaphylaxis.
Main Methods:
- Extraction of R from RES-rich organs using acetone.
- Determination of peptidic nature.
- In vitro testing on guinea pig uterine horns.
- Fractionation using high voltage paper electrophoresis.
- Concentration via acetone precipitation.
Main Results:
- Reticulin-M (R) was successfully extracted and isolated.
- The active component of R was identified as a basic peptide fraction.
- R demonstrated antianaphylactic activity in vitro.
- R was concentrated using acetone precipitation.
Conclusions:
- Reticulin-M (R) is a natural antianaphylactic peptidic factor.
- R may function as a cytokine.
- The study provides a foundation for further research into R's therapeutic potential.