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LPS-dependent interaction of Mac-2-binding protein with immobilized CD14
1Laboratory of Cellular Physiology and Immunology, Rockefeller University, New York, New York 10021, USA.
Journal of Inflammation
|January 1, 1995
Summary
Researchers identified Mac-2 binding protein (Mac-2-BP) as a novel plasma protein that binds to CD14 in the presence of lipopolysaccharide (LPS). This finding suggests Mac-2-BP may play a role in modulating cellular responses to bacterial endotoxins.
Area of Science:
- Immunology
- Biochemistry
- Cell Biology
Background:
- CD14 is a key receptor mediating cellular responses to bacterial lipopolysaccharide (LPS).
- LPS binding protein (LBP) facilitates LPS binding to CD14.
- The full spectrum of plasma proteins interacting with CD14 remains incompletely understood.
Purpose of the Study:
- To identify additional plasma proteins that bind to CD14.
- To investigate the functional role of newly identified CD14-binding proteins in LPS response.
Main Methods:
- Affinity chromatography using immobilized CD14 with and without LPS.
- Protein elution and identification.
- Functional assays using purified Mac-2-binding protein (Mac-2-BP) in cell-based LPS response models.
Main Results:
- LPS binding protein (LBP) and Mac-2-binding protein (Mac-2-BP) were identified as CD14-binding proteins in plasma.
- Mac-2-BP binding to CD14 required the presence of LPS.
- Purified Mac-2-BP did not induce or block LPS responses but slowed LPS neutralization by plasma lipoproteins.
Conclusions:
- Mac-2-BP is a novel CD14-binding protein involved in LPS interaction.
- Mac-2-BP may contribute to the regulation of LPS neutralization in plasma.
- These findings expand our understanding of the innate immune response to bacterial endotoxins.