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Plasma interleukin 8 and polymorphonuclear leukocyte elastase concentrations in patients with septic shock
1Critical Care and Emergency Center, Iwate Medical University, Morioka, Japan.
Abstract:
We determined the plasma concentrations of interleukin 8 (IL-8), polymorphonuclear leukocyte elastase (PMNE), and endotoxin in patients with septic shock in order to investigate the role of IL-8 and PMNE in the development of septic shock, especially in septic adult respiratory distress syndrome (ARDS). The IL-8 concentration in patients with septic shock was 6.28 +/- 9.00 ng/mL (mean +/- SD, n = 29), which was significantly higher (P < 0.0001) than the concentration in septic patients without shock (0.35 +/- 0.35 ng/mL, n = 40). There was a significant correlation between the IL-8 concentration and the PMNE concentration at the onset of septic shock (r = 0.6916, P < 0.0001). The IL-8 concentration was also significantly correlated with the endotoxin concentration (r = 0.5584, P = 0.0016). There was a significant negative correlation (r = -0.8237, P < 0.0001) between the serum PMNE concentration and the oxygenation index (PaO2/FiO2) at the onset of septic shock. These results indicate that IL-8 and PMNE are produced in large quantities when septic shock occurs, and may play a role in the development of septic ARDS.
Insights
Elevated levels of interleukin 8 (IL-8) and polymorphonuclear leukocyte elastase (PMNE) are linked to septic shock and adult respiratory distress syndrome (ARDS). These inflammatory markers correlate with disease severity and endotoxin levels.
Area of Science:
- Biochemistry
- Immunology
- Critical Care Medicine
Background:
- Septic shock is a life-threatening condition characterized by circulatory dysfunction.
- Adult respiratory distress syndrome (ARDS) is a common and severe complication of septic shock.
- The roles of specific inflammatory mediators in septic shock and ARDS require further elucidation.
Purpose of the Study:
- To investigate the plasma concentrations of interleukin 8 (IL-8) and polymorphonuclear leukocyte elastase (PMNE) in patients with septic shock.
- To determine the correlation between IL-8, PMNE, and endotoxin levels in septic shock.
- To explore the relationship between PMNE and the oxygenation index in septic shock.
Main Methods:
- Plasma concentrations of IL-8, PMNE, and endotoxin were measured in patients with and without septic shock.
- Statistical analyses, including correlation coefficients and significance testing, were employed.
- The oxygenation index (PaO2/FiO2) was assessed at the onset of septic shock.
Main Results:
- IL-8 concentrations were significantly higher in patients with septic shock compared to those without shock (P < 0.0001).
- Significant positive correlations were observed between IL-8 and PMNE (r = 0.6916, P < 0.0001) and between IL-8 and endotoxin (r = 0.5584, P = 0.0016).
- A significant negative correlation was found between serum PMNE and the oxygenation index (r = -0.8237, P < 0.0001).
Conclusions:
- IL-8 and PMNE are produced in substantial amounts during septic shock.
- These inflammatory mediators may contribute to the pathogenesis of septic shock and ARDS.
- The findings highlight the potential involvement of IL-8 and PMNE in the development of septic ARDS.