Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Differing alpha-tocopherol oxidative lability and ascorbic acid sparing effects in buoyant and dense LDL

D L Tribble1, P M Thiel, J J van den Berg

  • 1Department of Molecular and Nuclear Medicine, Lawrence Berkeley National Laboratory, Calif, USA.

Arteriosclerosis, Thrombosis, and Vascular Biology
|November 1, 1995
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bidirectional Mendelian randomization to explore the causal relationships between body mass index and polycystic ovary syndrome.

Human reproduction (Oxford, England)·2018
Same author

Pathways and mechanisms linking dietary components to cardiometabolic disease: thinking beyond calories.

Obesity reviews : an official journal of the International Association for the Study of Obesity·2018
Same author

New Pharmacogenomics Research Network: An Open Community Catalyzing Research and Translation in Precision Medicine.

Clinical pharmacology and therapeutics·2017
Same author

Candidate-Gene Study of Functional Polymorphisms in SLCO1B1 and CYP3A4/5 and the Cholesterol-Lowering Response to Simvastatin.

Clinical and translational science·2017
Same author

Effects of oxidants and antioxidants evaluated using parinaric acid as a sensitive probe for oxidative stress.

Redox report : communications in free radical research·2016
Same author

Direct electronic measurement of Peltier cooling and heating in graphene.

Nature communications·2016

Dense LDL depletes alpha-tocopherol faster than buoyant LDL due to lower antioxidant content. Ascorbic acid reverses this effect, highlighting its role in protecting LDL from oxidation.

Area of Science:

  • Lipid biochemistry
  • Oxidative stress research

Background:

  • Smaller, denser low-density lipoprotein (LDL) particles exhibit increased susceptibility to oxidation.
  • Lower levels of antioxidants, such as ubiquinol-10 and alpha-tocopherol, contribute to the enhanced oxidizability of dense LDL.

Purpose of the Study:

  • To investigate the differential rates of alpha-tocopherol depletion in dense versus buoyant LDL.
  • To examine the influence of ascorbic acid on the oxidation of LDL subfractions.

Main Methods:

  • Comparative analysis of alpha-tocopherol depletion rates in dense and buoyant LDL under varying copper (Cu2+) concentrations.
  • Assessment of conjugated diene formation as a marker of LDL oxidation.
  • Evaluation of the protective effect of ascorbic acid on LDL oxidation.

Related Experiment Videos

Main Results:

  • Dense LDL showed significantly faster alpha-tocopherol depletion compared to buoyant LDL, especially at low Cu2+ concentrations.
  • Ascorbic acid markedly extended the time to half-maximal alpha-tocopherol depletion in both LDL subfractions, with a more pronounced effect in dense LDL.
  • Differences in depletion rates between LDL subfractions were reversed in the presence of ascorbic acid.

Conclusions:

  • Dense LDL is more prone to rapid alpha-tocopherol depletion and oxidation, particularly when antioxidant defenses are limited.
  • Ascorbic acid plays a crucial role in protecting LDL from oxidation by recycling alpha-tocopherol, mitigating the subfraction-dependent differences.