[Effect of electric fields on the proteins in nerve regeneration conditioned fluid]

80 SD rats were randomly divided into four groups of 20 each: local electrostimulation of nerve stump (Group LS); electro-stimulation of myeloneure (Group N); electrostimulation of the denervated muscle (Group M); and controls (Group C). The left lateral sciatic nerve of rats was excised 5mm in length, and the severed nerve was bridged with the silicon tube. The gap between the stumps was about 10mm. At 3rd, 7th, 14th, 21st and 28th day after nerve transection and tube implantation, the fluid in the silicon tube was aspirated. The sample of fluid was spun and 4 microliters aliquots were taken from the supernatant. Then the electrophoresis of the aliquots was made by phase-system and assayed to the amount and variety by Gel Scan system. The results showed that the group LS had a high increase in the protein amount than others in the range of 6.16-10.23 x 10(3)D molecular weight. We believe that it is one of the mechanisms of the electric fields promoting nerve regeneration.