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Related Experiment Videos

Quantitative GLC determination of oxycodone in human plasma

N L Renzi, J N Tam

    Journal of Pharmaceutical Sciences
    |January 1, 1979
    PubMed
    Summary

    A new gas-liquid chromatography (GLC) assay accurately measures oxycodone in human plasma. This method is suitable for bioavailability studies, showing peak plasma levels after a standard dose.

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    Area of Science:

    • Pharmacokinetics
    • Analytical Chemistry

    Background:

    • Accurate quantification of oxycodone in biological matrices is crucial for therapeutic drug monitoring and clinical trials.
    • Existing analytical methods may require optimization for sensitivity and specificity in human plasma.

    Purpose of the Study:

    • To develop and validate a gas-liquid chromatography (GLC) assay for determining oxycodone concentrations in human plasma.
    • To utilize the developed assay in bioavailability studies of therapeutic oxycodone doses.

    Main Methods:

    • Development of a GLC assay utilizing a nitrogen-specific detector.
    • Analysis of human plasma samples from volunteers following oral administration of a commercial oxycodone tablet.

    Main Results:

    • The developed GLC assay demonstrated reliable determination of oxycodone levels in human plasma.
    • Following ingestion of a 4.5 mg oxycodone hydrochloride and 0.38 mg oxycodone terephthalate tablet, the mean peak plasma oxycodone concentration was 18.4 ng/ml at 1 hour in six volunteers.

    Conclusions:

    • The established GLC assay is effective for quantifying oxycodone in human plasma.
    • This method is suitable for assessing the bioavailability of therapeutic oxycodone doses in clinical pharmacokinetic studies.

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