Papaver bracteatum Lindl.: quantitative extraction and determination of thebaine

An interlaboratory procedure for the quantitative extraction and analysis of thebaine from different tissues of Papaver bracteatum Lindl. is presented. Each step was evalutated for the yield of thebaine by use of 1-3H-thebaine and GLC. The method of drying and milling of tissue and the size of resultant particles were important factors in the quantitative recovery of thebaine.