Two endogenous methyl-alpha-D-glucopyranoside uptake activities in Xenopus oocytes

Endogenous methyl-alpha-D-glucopyranoside (AMG) uptake in Xenopus oocytes was measured with 14C-labeled AMG. Two AMG uptake activities, one Na(+)-dependent and the other Na(+)-independent, were observed in oocytes incubated with 2 mM AMG. However, only Na(+)-dependent uptake was observed at 0.1 mM AMG. Na(+)-dependent AMG uptake was attributed to the endogenous Na+/glucose co-transporter since it was inhibited by phlorizin. On the other hand, Na(+)-independent AMG uptake was inhibited by cytochalasin B or 2-deoxy-D-glucose. This suggests that AMG can be transported by the endogenous facilitated glucose transporter as well as by the endogenous co-transporter. In addition, a substrate range of the endogenous co-transporter was examined by competition experiments.