Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jul 1, 2026

Transplantation of GFP-expressing Blastomeres for Live Imaging of Retinal and Brain Development in Chimeric Zebrafish Embryos

Transplantation of GFP-expressing Blastomeres for Live Imaging of Retinal and Brain Development in Chimeric Zebrafish Embryos

Published on: July 20, 2010

Gridlock, a localized heritable vascular patterning defect in the zebrafish

B M Weinstein¹, D L Stemple, W Driever

¹Cardiovascular Research Center, Massachusetts General Hospital, Charlestown 02129, USA.

Nature Medicine

|November 1, 1995

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Emergence of Functional Heart-Brain Circuits in a Vertebrate.

bioRxiv : the preprint server for biology·2025

Same author

Imaging blood vessels and lymphatic vessels in the zebrafish.

Methods in cell biology·2016

Same author

Oct4/Pou5f1 controls tissue-specific repressors in early zebrafish embryo.

Journal of stem cells & regenerative medicine·2014

Same author

The zebrafish as a model system to study cardiovascular development.

Trends in cardiovascular medicine·2011

Same author

A genetic screen for vascular mutants in zebrafish reveals dynamic roles for Vegf/Plcg1 signaling during artery development.

Developmental biology·2009

Same author

XuvTools: free, fast and reliable stitching of large 3D datasets.

Journal of microscopy·2009

Same journal

Generalizable AI predicts immunotherapy outcomes across cancers and treatments.

Nature medicine·2026

Same journal

Immune aging biomarkers for clinical trials.

Nature medicine·2026

Same journal

Lassa fever countermeasures gather pace.

Nature medicine·2026

Same journal

Why high scores do not mean application readiness for health AI.

Nature medicine·2026

Same journal

Polypill for heart failure with reduced ejection fraction: the POLY-HF randomized trial.

Nature medicine·2026

Same journal

Biological aging might help to explain the rising risk of early-onset cancer.

Nature medicine·2026

See all related articles

A zebrafish mutation, gridlock (grl), causes a vascular defect, impeding tail blood flow. Compensatory collateral vessels develop, bypassing the blockage and enabling survival.

Area of Science:

Developmental biology
Genetics
Cardiovascular research

Background:

The development and patterning of the vertebrate vasculature are complex processes involving numerous genes.
Congenital cardiovascular malformations, such as coarctation of the aorta, affect many newborns and have unknown etiologies.

Purpose of the Study:

To identify genes involved in vertebrate vascular development using the zebrafish model.
To characterize a novel mutation affecting blood flow and vascular patterning.

Main Methods:

Isolation and characterization of a recessive zebrafish mutation, gridlockm145 (grlm145).
Utilizing a novel microangiographic technique to visualize vascular defects.
Analyzing the development of collateral vessels and arterial-venous shunts in mutant embryos.

More Related Videos

Mechanical Vessel Injury in Zebrafish Embryos

Mechanical Vessel Injury in Zebrafish Embryos

Published on: February 17, 2015

Light-sheet Fluorescence Microscopy to Capture 4-Dimensional Images of the Effects of Modulating Shear Stress on the Developing Zebrafish Heart

Light-sheet Fluorescence Microscopy to Capture 4-Dimensional Images of the Effects of Modulating Shear Stress on the Developing Zebrafish Heart

Published on: August 10, 2018

Related Experiment Videos

Last Updated: Jul 1, 2026

Transplantation of GFP-expressing Blastomeres for Live Imaging of Retinal and Brain Development in Chimeric Zebrafish Embryos

Transplantation of GFP-expressing Blastomeres for Live Imaging of Retinal and Brain Development in Chimeric Zebrafish Embryos

Published on: July 20, 2010

Mechanical Vessel Injury in Zebrafish Embryos

Mechanical Vessel Injury in Zebrafish Embryos

Published on: February 17, 2015

Light-sheet Fluorescence Microscopy to Capture 4-Dimensional Images of the Effects of Modulating Shear Stress on the Developing Zebrafish Heart

Light-sheet Fluorescence Microscopy to Capture 4-Dimensional Images of the Effects of Modulating Shear Stress on the Developing Zebrafish Heart

Published on: August 10, 2018

Main Results:

The grlm145 mutation causes a localized vascular defect in the anterior trunk, obstructing blood flow to the tail.
This defect occurs at the site where paired lateral dorsal aortae normally merge into the single midline aorta.
Mutant embryos develop arterial-venous shunts and collateral vessels to bypass the lesion, restoring caudal blood flow.

Conclusions:

The zebrafish gridlock mutation provides a model for studying vascular patterning defects.
The grl mutation's phenotype resembles human coarctation of the aorta, offering insights into this congenital malformation.
The compensatory development of collateral vessels highlights adaptive mechanisms in cardiovascular development.