Related Experiment Video

Updated: Jul 3, 2026

A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA
06:53

A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA

Published on: December 26, 2015

A single-injection protein kinase A-directed antisense treatment to inhibit tumour growth

M Nesterova1, Y S Cho-Chung

  • 1Cellular Biochemistry Section, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-1750, USA.

Nature Medicine
|June 1, 1995
PubMed

Insights

Inhibition of RI alpha gene expression using antisense treatment reduced tumor growth in vivo. This approach effectively controlled neoplastic growth by restoring normal cellular behavior.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • The RI alpha subunit of cAMP-dependent protein kinase type I is upregulated in various cancer types and during cell growth.
  • Understanding the role of RI alpha in neoplastic growth is crucial for developing targeted cancer therapies.

Purpose of the Study:

  • To investigate the impact of sequence-specific inhibition of RI alpha gene expression on in vivo tumor growth.
  • To determine if targeting RI alpha can modulate neoplastic cell behavior.

Main Methods:

  • RI alpha antisense treatment was administered via single injection to induce sequence-specific gene silencing.
  • Tumor growth and RI alpha expression levels were monitored in vivo following treatment.

Main Results:

  • Single-injection RI alpha antisense treatment led to a significant reduction in RI alpha expression.
  • This reduction in RI alpha expression resulted in the inhibition of in vivo tumor growth.
  • Tumor cells treated with RI alpha antisense exhibited characteristics similar to untransformed cells, producing less protein kinase type I.

Conclusions:

  • Sequence-specific inhibition of RI alpha gene expression is a viable strategy for controlling in vivo tumor growth.
  • RI alpha antisense treatment offers a potential therapeutic approach for neoplastic diseases, requiring infrequent dosing for sustained effect.

More Related Videos

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays13:22

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays

Published on: October 23, 2019

Efficient Gene Knockdown in the Liver via Intrasplenic Injection of Adeno-Associated Virus Serotype 8 (AAV8)-Delivered Small Hairpin RNA04:29

Efficient Gene Knockdown in the Liver via Intrasplenic Injection of Adeno-Associated Virus Serotype 8 (AAV8)-Delivered Small Hairpin RNA

Published on: November 1, 2024

Related Concept Videos

RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against specific...
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a rapamycin-insensitive companion...
Inhibitors of Viral Protein Synthesis01:30

Inhibitors of Viral Protein Synthesis

Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...