Interaction between calcium and zinc on L-threonine absorption in rabbit jejunum

The essential minerals calcium and zinc serve unique functions in higher organisms, and it is well recognized that homeostatic mechanisms are involved in regulating their metabolism. However, it has been reported that zinc, at higher concentrations (1 mM), inhibits intestinal absorption of sugars and amino acids. The aim of the present work was to determine whether the inhibitory effect on L-threonine absorption across the rabbit jejunum could be modified by calcium. In media with Ca2+, zinc significantly reduced L-threonine absorption. In Ca(2+)-free media, where calcium chloride was omitted and replaced isotonically with choline chloride, the amino acid transport was not modified by zinc, but when calcium chloride was replaced isotonically with magnesium chloride, the inhibition was observed. Verapamil (blocking mainly Ca2+ transport) did not modify the inhibitory effect of zinc on L-threonine transport. When A23187 (Ca(2+)-specific ionophore) was added in media with and without Ca2+, zinc produced no change in L-threonine transport. These results suggest that calcium and zinc could have an affinity with the same chemical groups of the enterocyte membrane, which would be related to the intestinal absorption of amino acids.