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Subcellular location of neutrophil opsonic receptors is altered by exogenous reactive oxygen species

H H Simms1, R D'Amico

  • 1Department of Surgery, Brown University School of Medicine/Rhode Island Hospital, Providence 02903, USA.

Cellular Immunology
|November 1, 1995
PubMed

Insights

Exposure to extracellular oxidants causes polymorphonuclear neutrophils (PMN) to relocate Fc gamma and complement receptors within the cell. This redistribution of opsonic receptors alters PMN function, impacting immune responses.

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Polymorphonuclear neutrophils (PMN) play a crucial role in innate immunity.
  • The oxidative burst in PMNs is critical for pathogen clearance.
  • Subcellular localization of receptors influences PMN function.

Purpose of the Study:

  • To investigate the effect of extracellular oxidants on the subcellular localization of key PMN receptors.
  • To determine how oxidant-induced receptor redistribution impacts PMN function.

Main Methods:

  • PMNs were incubated with glucose/glucose oxidase or xanthine/xanthine oxidase to generate extracellular oxidants.
  • Subcellular fractions were analyzed for the presence of Fc gamma receptors (CD64, CD32w, CD16) and complement receptors (CD35, CD11b/CD18).
  • Functional assays were performed to assess receptor-mediated PMN activity.

Main Results:

  • Extracellular oxidants induced intracellular shifts of Fc gamma receptors and altered complement receptor expression.
  • Receptor redistribution was blocked by antioxidants (catalase, superoxide dismutase) and cytoskeleton stabilizers (phalloidin, Taxol).
  • Involvement of specific signaling pathways (H-7, staurosporine, genistein) was identified.
  • Relocation of receptors to the plasma membrane correlated with increased functional activity, such as IgG and complement binding.

Conclusions:

  • Extracellular oxidant exposure triggers a coordinated subcellular relocation of opsonic receptors in PMNs.
  • This receptor redistribution is a regulated process involving the cytoskeleton and specific signaling molecules.
  • Oxidant-induced receptor relocation significantly modulates PMN functional responses, highlighting a novel mechanism in immune cell activation.

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