Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Altered selenium-binding protein levels associated with selenium resistance

L Wu1, L McGarry, J Lanfear

  • 1Beatson Institute for Cancer Research, CRC Beatson Laboratories, Wolfson Laboratories for Molecular Pathology, Bearsden, Glasgow, UK.

Carcinogenesis
|November 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Strong Opioid Use after Total Knee Arthroplasty.

Irish medical journal·2024
Same author

Determination of specular reflectances in a liquid medium with a variable angle of incidence.

Applied optics·2010
Same author

p120-catenin is required for the collective invasion of squamous cell carcinoma cells via a phosphorylation-independent mechanism.

Oncogene·2007
Same author

A randomized trial of controlled-release oxycodone during inpatient rehabilitation following unilateral total knee arthroplasty.

The Journal of bone and joint surgery. American volume·2001
Same author

Regulation of a multigenic invasion programme by the transcription factor, AP-1: re-expression of a down-regulated gene, TSC-36, inhibits invasion.

Oncogene·2000
Same author

Transcriptional regulation of cell invasion: AP-1 regulation of a multigenic invasion programme.

European journal of cancer (Oxford, England : 1990)·2000
Same journal

Correction to: Hypoxia-increased RAGE and P2X7R expression regulates tumor cell invasion through phosphorylation of Erk1/2 and Akt and nuclear translocation of NF-κB.

Carcinogenesis·2026
Same journal

Correction: Arenobufagin, a natural bufadienolide from toad venom, induces apoptosis and autophagy in human hepatocellular carcinoma cells through inhibition of PI3K/Akt/mTOR pathway.

Carcinogenesis·2026
Same journal

Wnt/β-catenin Signaling in Hepatocellular Carcinoma: A Major Context-Dependent Contributor to Resistance to Tyrosine Kinase Inhibitors and Immune Checkpoint Inhibitors.

Carcinogenesis·2026
Same journal

TGF-β1 and TGF-β2 family members differentially modulate tumor initiation and invasiveness of primary liver cancer in a MMP14-dependent manner.

Carcinogenesis·2026
Same journal

MEK/ERK/RSK2 positive feedback loop modulates EMT to promote migration and invasion of triple-negative breast cancer through regulating GALNT5 mRNA stability.

Carcinogenesis·2026
Same journal

ADH1B-ALDH2 genotype combinations, Age-Related Risk, and Field Cancerization in Head and Neck and Upper GI Cancer Screening in 10,073 Alcohol-Dependent Men.

Carcinogenesis·2026
See all related articles

Selenium resistance in cancer cells may stem from changes in specific selenium-binding proteins. Researchers identified two novel 72 kDa proteins in resistant cells, suggesting a new pathway for selenium

Area of Science:

  • Biochemistry
  • Cell Biology
  • Cancer Research

Background:

  • Selenium's chemopreventive properties are linked to its cell growth inhibition mechanisms.
  • A selenium-resistant cell line (B19) was previously isolated from a selenium-sensitive parental line (C57).

Purpose of the Study:

  • To identify biochemical changes conferring selenium resistance in the B19 cell line.
  • To understand the role of selenium-binding proteins in cellular response to selenium.

Main Methods:

  • Comparison of selenium uptake and intracellular glutathione concentrations between B19 and C57 cells.
  • Proteomic analysis using [75Se]selenite labeling to compare selenium-binding proteins.
  • Analysis of mRNA levels for antioxidant and detoxification enzymes.

Related Experiment Videos

Main Results:

  • Selenium uptake and glutathione levels were similar in both cell lines, ruling them out as resistance mechanisms.
  • Significant differences in selenium-binding proteins were observed between B19 and C57 cells upon [75Se]selenite labeling.
  • Two novel 72 kDa selenium-binding proteins were uniquely detected in the resistant B19 cells.

Conclusions:

  • Altered expression of specific selenium-binding proteins, particularly the 72 kDa proteins, likely contributes to selenium resistance.
  • Further investigation of these 72 kDa proteins may elucidate selenium's growth inhibition mechanisms.
  • Findings provide insights into cellular adaptation to selenium exposure and potential chemopreventive strategies.