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Related Experiment Videos

Quantitative cleaning efficacy evaluation methods with protein and lipid lens soiling models

J Hu1, F Jara, J Thakrar

  • 1Pilkington Barnes Hind, Sunnyvale, CA 94086, USA.

The CLAO Journal : Official Publication of the Contact Lens Association of Ophthalmologists, Inc
|July 1, 1995
PubMed
Summary

This study introduces a new method to quantitatively assess rigid gas permeable contact lens cleaners. Both tested systems effectively removed protein and lipid deposits, proving superior to water and saline controls.

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Area of Science:

  • Ophthalmology
  • Biomaterials Science
  • Analytical Chemistry

Background:

  • Rigid gas permeable (RGP) contact lenses require effective cleaning systems to remove protein and lipid deposits.
  • Existing methods for evaluating cleaner efficacy may lack quantitative precision.
  • Development of a reliable, nondestructive method is crucial for RGP lens care product assessment.

Purpose of the Study:

  • To develop and validate a nondestructive, quantitative method for assessing the cleaning efficacy of RGP contact lens care systems.
  • To compare the performance of specific commercial RGP lens care systems against controls.

Main Methods:

  • Simulated tear proteins (lysozyme, albumin, calcium) and a lipid mixture (grease, unsaturated fat) were used to soil RGP lenses.
  • Protein deposits were quantified using spectrophotometry (absorbance at 280 nm).

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  • Lipid deposits were quantified using fluorescence spectrophotometry (absorbance at 430 nm).

    • Main Results:

    • Both ComfortCare and Boston Advance systems showed significantly higher cleaning efficacy than water (protein) and saline (lipid) controls.
    • The developed method provided quantitative measurements for both protein and lipid removal.
    • Statistical analysis confirmed the significant differences in cleaning performance.

    Conclusions:

    • The developed method offers a reliable, quantitative, and nondestructive approach for evaluating RGP contact lens cleaner efficacy.
    • Commercial lens care systems demonstrate significant effectiveness in removing common RGP lens deposits.
    • This methodology is suitable for screening and comparative analysis of lens care products.