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Composition of interstitial fluid

N Fogh-Andersen1, B M Altura, B T Altura

  • 1Department of Clinical Chemistry, Herlev Hospital, Denmark.

Clinical Chemistry
|October 1, 1995
PubMed
Summary
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This study estimates interstitial fluid composition by measuring plasma changes after subjects lie down. Results reveal lower protein, calcium, and magnesium concentrations in interstitial fluid compared to plasma.

Area of Science:

  • Physiology
  • Biochemistry
  • Fluid Dynamics

Background:

  • Previous interstitial fluid collection methods yielded inconsistent results.
  • Electrolyte concentrations in interstitial fluid often deviate from plasma ultrafiltrate.
  • Accurate interstitial fluid composition is crucial for understanding fluid exchange and physiological processes.

Purpose of the Study:

  • To determine the composition of interstitial fluid using a novel hemodilution method.
  • To quantify protein, electrolyte, and cell concentrations in interstitial fluid.
  • To investigate the implications of interstitial fluid composition on endothelial transport and cell dynamics.

Main Methods:

  • Measuring changes in hematocrit and plasma composition in 20 subjects before and after assuming a supine position.

Related Experiment Videos

  • Utilizing the hemodilution effect caused by fluid shift from interstitial to plasma compartments.
  • Calculating interstitial fluid composition based on observed plasma dilution and known fluid shifts.

    • Main Results:

    • Estimated protein concentration in interstitial fluid was 20.6 g/L.
    • Lower concentrations of total calcium and magnesium were observed in interstitial fluid.
    • Reduced activity of free cations and a Donnan equilibrium potential of 1 mV across the endothelium were noted.
    • Leukocyte and platelet concentrations decreased, indicating no significant escape or mobilization.

    Conclusions:

    • The hemodilution method provides a reliable estimation of interstitial fluid composition.
    • Interstitial fluid has a lower protein concentration than plasma, affecting calcium and magnesium binding.
    • Endothelial transport mechanisms and cell dynamics are influenced by interstitial fluid composition.