Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Developmental signalling

I A Hendry1, S O Johanson, K Heydon

  • 1Division of Neuroscience, John Curtin School of Medical Research, Australian National University, Canberra, Australia.

Clinical and Experimental Pharmacology & Physiology
|August 1, 1995
PubMed
Summary

Neurotrophic factors communicate with neurons via self-transport or second messengers. Gz alpha, a GTP-binding protein, is retrogradely transported to the neuron cell body nucleus, suggesting its role in neuronal signaling.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Absence of binding of alpha-bungarotoxin and cobra neurotoxin to central acetylcholine receptors - an autoradiographic study.

Neuroscience letters·2009
Same author

Parasympathetic neuronal survival induced by factors from muscle.

Neuroscience letters·2009
Same author

Deletion of guanine nucleotide binding protein alpha z subunit in mice induces a gene dose dependent tolerance to morphine.

Neuropharmacology·2004
Same author

Comparison of nerve terminal events in vivo effecting retrograde transport of vesicles containing neurotrophins or synaptic vesicle components.

Journal of neuroscience research·2004
Same author

What is the importance of multivesicular bodies in retrograde axonal transport in vivo?

Journal of neurobiology·2004
Same author

Loss of p16 expression is of prognostic significance in locally advanced prostate cancer: an analysis from the Radiation Therapy Oncology Group protocol 86-10.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology·2003

Area of Science:

  • Neuroscience
  • Cell Biology
  • Molecular Biology

Background:

  • Neuronal communication relies on neurotrophic factors.
  • Two classes of factors exist: self-transporting and second messenger-dependent.
  • Stable second messengers are crucial for factors transported to the cell body.

Purpose of the Study:

  • To investigate the communication pathways between target tissue and neurons.
  • To identify stable second messengers involved in retrograde neuronal transport.
  • To explore the role of GTP-binding proteins in neuronal signaling.

Main Methods:

  • Investigated retrograde transport of neurotrophic factors in mouse sciatic nerve.
  • Examined the localization of Gz alpha in dorsal root ganglia cell bodies.
  • Utilized axonal ligation to assess changes in Gz alpha immunoreactivity.

Main Results:

  • GTP-binding proteins, specifically Gi alpha and Gz alpha, undergo retrograde transport.
  • Gz alpha accumulates in the nucleus of dorsal root ganglia cells with intact axons.
  • Axonal ligation leads to decreased Gz alpha immunoreactivity in cell bodies within 24 hours.

Conclusions:

  • Gz alpha is a potential stable second messenger.
  • Gz alpha is activated at the nerve terminal and transported to the cell body.
  • Nuclear accumulation of Gz alpha in cell bodies suggests a role in neuronal signaling and survival.

Related Experiment Videos