A comparative study of orientation at behavior of univalent in living grasshopper spermatocytes

Orientational movements and modes of segregation at anaphase I were analyzed in three different types of univalents in living spermatocytes of the grasshopper species Eyprepocnemis plorans, namely the sex univalent, three types of accessory chromosomes and spontaneous and induced autosomal univalents. When two or more univalents were present in the same spindle, their dynamics were directly compared. Chromosomes may show variable velocity and number of reorientations: the X and the most common B types (B1 and B2) are slow and rarely reorient, a more geographically restricted B (B5) is faster and reorients more often, and autosomal univalents are the fastest and show the highest frequency of reorientations. Nonetheless, the X and the accessories are rigorously reductional at anaphase I whereas autosomal univalents often fail to migrate or divide equationally. This indicates that orientational and segregational behavior are controlled mainly by chromosomal rather than cellular characteristics and that chromosomes may display a great variety of strategies to achieve regular segregation.