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Related Experiment Videos

Intercellular calcium waves mediated by inositol trisphosphate

M J Sanderson1

  • 1Department of Anatomy and Cell Biology, UCLA School of Medicine 90024-1763, USA.

Ciba Foundation Symposium
|January 1, 1995
PubMed
Summary
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Mechanical stimulation triggers intercellular calcium waves via inositol 1,4,5-trisphosphate (InsP3) diffusion through gap junctions, not calcium itself. This cell communication is crucial in epithelial and glial cells.

Area of Science:

  • Cell Biology
  • Biophysics
  • Signal Transduction

Background:

  • Intercellular calcium waves are observed in various cell types, particularly epithelial and glial cells.
  • Mechanical stimulation of a single cell increases intracellular free calcium ([Ca2+]i), initiating a wave that spreads within the cell.

Purpose of the Study:

  • To investigate the mechanism of intercellular calcium wave propagation induced by mechanical stimulation.
  • To determine the role of inositol 1,4,5-trisphosphate (InsP3) and gap junctions in wave propagation.

Main Methods:

  • Mechanical stimulation of individual cells.
  • Measurement of intracellular calcium concentration ([Ca2+]i).
  • Assessment of gap junction function, extracellular calcium presence, and inhibition using heparin, U73122, and thapsigargin.

Related Experiment Videos

  • Mathematical modeling of InsP3 diffusion.

    • Main Results:

    • Intercellular calcium waves propagate through adjacent cells after an initial intracellular wave.
    • Wave propagation requires functional gap junctions and is independent of extracellular calcium.
    • InsP3 microinjection triggers waves, while InsP3 receptor antagonists (heparin) and phospholipase C inhibitors (U73122) block propagation.
    • Depletion of intracellular calcium stores also inhibits wave propagation.

    Conclusions:

    • Mechanical stimulation induces InsP3 production, which then propagates intercellular calcium waves through gap junctions.
    • Inositol 1,4,5-trisphosphate (InsP3) acts as the primary messenger between cells, not calcium ions.
    • Mathematical models support InsP3 diffusion as a viable mechanism for intercellular calcium wave generation.