Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calcium oscillations in neurons

D D Friel1

  • 1Department of Neurosciences, Case Western Reserve University, Cleveland, OH 44106-4975, USA.

Ciba Foundation Symposium
|January 1, 1995
PubMed
Summary
This summary is machine-generated.

Caffeine triggers calcium oscillations in sympathetic neurons by interacting with internal stores and the plasma membrane. This process involves calcium release and entry, creating cyclic changes in cytosolic calcium levels.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multiple modes of calcium-induced calcium release in sympathetic neurons I: attenuation of endoplasmic reticulum Ca2+ accumulation at low [Ca2+](i) during weak depolarization.

The Journal of general physiology·2001
Same author

Multiple modes of calcium-induced calcium release in sympathetic neurons II: a [Ca2+](i)- and location-dependent transition from endoplasmic reticulum Ca accumulation to net Ca release.

The Journal of general physiology·2001
Same author

Mitochondria as regulators of stimulus-evoked calcium signals in neurons.

Cell calcium·2000
Same author

Dissection of mitochondrial Ca2+ uptake and release fluxes in situ after depolarization-evoked [Ca2+](i) elevations in sympathetic neurons.

The Journal of general physiology·2000
Same author

Quantitative analysis of mitochondrial Ca2+ uptake and release pathways in sympathetic neurons. Reconstruction of the recovery after depolarization-evoked [Ca2+]i elevations.

The Journal of general physiology·2000
Same author

Depolarization-induced mitochondrial Ca accumulation in sympathetic neurons: spatial and temporal characteristics.

The Journal of neuroscience : the official journal of the Society for Neuroscience·1999

Area of Science:

  • Cellular Physiology
  • Neuroscience
  • Calcium Signaling

Background:

  • Cytosolic free Ca2+ concentration ([Ca2+]i) oscillations occur in various cell types.
  • These oscillations can be driven by membrane potential changes, internal Ca2+ stores, or a combination of factors.

Purpose of the Study:

  • To investigate the mechanisms of caffeine-induced [Ca2+]i oscillations in sympathetic neurons.
  • To elucidate the roles of the plasma membrane and caffeine-sensitive internal stores in these oscillations.

Main Methods:

  • Studied caffeine-induced [Ca2+]i oscillations in sympathetic neurons.
  • Examined the effects of ryanodine and external Ca2+ manipulation.
  • Analyzed the rate of change of [Ca2+]i (d[Ca2+]i/dt) to estimate Ca2+ fluxes.

Related Experiment Videos

Main Results:

  • Caffeine-induced oscillations occur at a steady membrane potential and are ryanodine-sensitive, indicating Ca2+-induced Ca2+ release.
  • External Ca2+ entry is crucial except during the rapid upstroke, which is dominated by internal store release.
  • Oscillations result from cyclic Ca2+ exchange between the cytosol and a caffeine-sensitive store.

Conclusions:

  • Caffeine-induced [Ca2+]i oscillations in sympathetic neurons are driven by the interplay between plasma membrane Ca2+ transport and a caffeine-sensitive internal store.
  • These oscillations represent cyclic perturbations of [Ca2+]i due to store Ca2+ exchange.
  • The study provides insights into the dynamics of Ca2+ release and entry during these neuronal oscillations.