Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calcium signalling during mammalian fertilization

S Miyazaki1

  • 1Department of Physiology, Tokyo Women's Medical College, Japan.

Ciba Foundation Symposium
|January 1, 1995
PubMed
Summary

Mammalian egg calcium (Ca2+) signaling involves waves and oscillations, primarily mediated by inositol 1,4,5-trisphosphate (InsP3) receptors. This Ca2+ signaling is crucial for egg activation and fertilization.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Isolation and characterization of type 1 fimbriae from a chicken pathogenic Escherichia coli serotype O78.

The Journal of veterinary medical science·1992
Same author

Treatment of Langerhans cell histiocytosis in children with etoposide.

American journal of clinical oncology·1992
Same author

[Hybridoma Data Bank (HDB)].

Nihon rinsho. Japanese journal of clinical medicine·1992
Same author

[Changes in serotypes of clinical isolates of Pseudomonas aeruginosa by anti-pseudomonal drugs].

Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases·1992
Same author

Expression of p53 in human esophageal carcinoma: an immunohistochemical study with correlation to proliferating cell nuclear antigen expression.

Human pathology·1992
Same author

Pathogenesis, treatment and prognosis of impending myocardial infarction and early post-infarction angina--relation between ST-segment shift during myocardial ischemia and the pathogenesis.

Japanese circulation journal·1992

Area of Science:

  • Cellular biology
  • Reproductive biology
  • Biochemistry

Background:

  • The fertilized mammalian egg serves as a model for studying spatiotemporal calcium (Ca2+) signaling.
  • Hamster eggs exhibit Ca2+ transients and waves originating from sperm attachment sites.

Purpose of the Study:

  • To analyze spatiotemporal Ca2+ signaling in fertilized and unfertilized mammalian eggs.
  • To investigate the role of inositol 1,4,5-trisphosphate (InsP3) receptors and other channels in Ca2+ dynamics.

Main Methods:

  • Utilized hamster eggs as a model system.
  • Induced Ca2+ waves and oscillations via injection of InsP3 or Ca2+.
  • Employed a monoclonal antibody against the type 1 InsP3 receptor to assess its role.
  • Performed physiological and immunoblot analyses to detect ryanodine receptors.

Main Results:

  • Ca2+ waves and oscillations were observed in both fertilized and unfertilized eggs.
  • These Ca2+ events were dose-dependently inhibited by an antibody to the type 1 InsP3 receptor.
  • Ryanodine receptors were not detected.
  • Positive and negative feedback loops involving cytosolic Ca2+ and InsP3-sensitive pools regulate signaling.
  • Extracellular Ca2+ influx, potentially mediated by inositol 1,3,4,5-tetrakisphosphate, is necessary for refilling Ca2+ pools.

Conclusions:

  • Spatiotemporal Ca2+ signaling in mammalian eggs is primarily mediated by InsP3 receptors.
  • Both intracellular Ca2+ release and influx are essential for maintaining Ca2+ oscillations.
  • Further research is needed to elucidate the upstream signaling pathways and downstream egg activation mechanisms.

Related Experiment Videos