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P450aldo in hamster adrenal cortex: immunofluorescent and immuno-gold electron microscopic studies
J G LeHoux1, D Martel, J LeHoux
1Department of Biochemistry, Faculty of Medicine, Sherbrooke University, QC, Canada.
Endocrine Research
|February 1, 1995
Abstract:
The zonal distribution of aldosterone synthase cytochrome P450 (P450aldo) in the adrenal cortex of male hamsters was investigated by immunofluorescence and electron microscopy, using an anti-P450aldo peptide antibody. On cryostat sections the immunolocalization of P450aldo was confined to the zona glomerulosa cells. On semi-thin plastic sections, P450aldo was shown to be located in mitochondria. Studies in electron microscopy, using the colloidal gold technique, confirmed that P450aldo was located in mitochondria.