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Related Experiment Videos

Cyclosporin A, rapamycin and FK506 decrease prolactin release from rat pituitary cells in primary culture

S Wera1, L Zheng, E L Hooghe-Peters

  • 1Department of Pharmacology, Vrije Universiteit Brussel, Belgium.

Endocrine Research
|August 1, 1995
PubMed
Summary

Immunosuppressant drugs like cyclosporin A, FK506, and rapamycin were tested for their effect on prolactin release. These drugs were found to inhibit prolactin production in rat pituitary cells, suggesting a role in their immunosuppressive action.

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Area of Science:

  • Endocrinology
  • Immunology
  • Pharmacology

Background:

  • Prolactin is known to have a general immunoactivating function.
  • The impact of common immunosuppressant drugs on prolactin release is not well understood.

Purpose of the Study:

  • To investigate whether cyclosporin A, FK506, and rapamycin affect prolactin release from rat pituitary cells.
  • To determine if these immunosuppressants influence prolactin production or secretion.

Main Methods:

  • Primary rat pituitary cell cultures were utilized.
  • Cells were incubated with varying concentrations of cyclosporin A, FK506, and rapamycin for short (2h) and long (48h) durations.
  • Prolactin levels in the culture medium were measured to assess release and production.

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Main Results:

  • Short-term incubation (2h) showed no significant effect of the drugs on prolactin release.
  • Long-term incubation (48h) with cyclosporin A, FK506, and rapamycin significantly reduced prolactin release (56-64%).
  • No cytotoxic effects were observed at the tested drug concentrations.

Conclusions:

  • Cyclosporin A, FK506, and rapamycin inhibit prolactin production, not just release from intracellular stores.
  • The inhibition of pituitary prolactin synthesis may be a contributing mechanism to the overall immunosuppressive effects of these drugs.