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Microchip electrophoresis with sample stacking

S C Jacobson1, J M Ramsey

  • 1Chemical and Analytical Sciences Division, Oak Ridge National Laboratory, TN 37831-6142, USA.

Electrophoresis
|April 1, 1995
PubMed
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This study presents a novel fused quartz microchip for rapid electrophoresis of dansylated amino acids. The microchip achieves fast separations with high reproducibility and detection limits using a stacking injection technique.

Area of Science:

  • Analytical Chemistry
  • Microfluidics
  • Separation Science

Background:

  • Microchip electrophoresis offers miniaturized and rapid separation capabilities.
  • Dansylated amino acids are important analytes in biological and chemical analysis.
  • Improving detection limits and separation speed in microchip electrophoresis is crucial.

Purpose of the Study:

  • To fabricate a fused quartz microchip with serpentine geometry for rapid electrophoresis.
  • To develop a stacking injection technique for enhanced detection limits.
  • To evaluate the performance of the microchip for dansylated amino acid separation.

Main Methods:

  • Fabrication of a serpentine column microchip on a quartz substrate using photolithography, etching, and deposition.

Related Experiment Videos

  • Precise control of buffer and sample flow via applied potentials.
  • Implementation of a stacking injection technique for sample pre-concentration.

    • Main Results:

    • Achieved rapid separations of dansylated amino acids in under 15 seconds.
    • Demonstrated high reproducibility of stacked injections (2.1% RSD in peak area).
    • Generated approximately 40,000 theoretical plates with a 67 mm separation length and 1100 V/cm field strength.

    Conclusions:

    • The developed fused quartz microchip enables fast and efficient separation of dansylated amino acids.
    • The stacking injection technique significantly enhances detection limits.
    • This microchip electrophoresis system shows great promise for high-throughput amino acid analysis.