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Structural elucidation of the O-antigenic polysaccharide from Escherichia coli O44:H18
M Staaf1, G Widmalm, A Weintraub
1Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Sweden.
European Journal of Biochemistry
|October 15, 1995
Abstract:
The O-antigen polysaccharide of the lipopolysaccharide from the enteroaggregative Escherichia coli O44:H18 has been investigated. Sugar and methylation analysis, 1H- and 13C-NMR spectroscopy revealed that the polysaccharide is composed of pentasaccharide repeating units. The sequence of sugar residues was determined by use of two-dimensional nuclear Overhauser effect spectroscopy and heteronuclear multiple bond correlation experiments. The structure of the repeating unit of the O-antigen from Escherichia coli O44:H18 is as follows. [formula: see text]