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Updated: Aug 2, 2026

Colony Forming Cell (CFC) Assay for Human Hematopoietic Cells
Published on: December 18, 2010
Hematopoietic reserve provided by spleen colony-forming units (CFU-S)
1Department of Pathophysiology, First Medical Faculty, Charles University, Prague, Czech Republic.
Abstract:
Using quantitative data available from the literature on murine hematopoiesis, the functional reserve of multipotential stem cells was calculated by comparing daily blood cell production with the potential of clonogenic spleen colony-forming cells (CFU-S) to generate blood cells. The potential of the day-8 CFU-S (CFU-S-8) population is estimated to be from 4000 to 37,000 times greater than needed in steady-state hematopoiesis. The CFU-S population may thus serve to provide a functional reserve to supply large numbers of peripheral blood cells via committed lineage-specific cells within days, whenever needed.
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