Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Progesterone induces human sperm chemotaxis

C Villanueva-Díaz1, J Arias-Martínez, L Bermejo-Martínez

  • 1Departamento de Fisiología de la Reproducción, Instituto Nacional de Perinatología, Mexico, D.F., Mexico.

Fertility and Sterility
|December 1, 1995
PubMed
Summary

Human follicular fluid contains a lipid-like molecule that attracts sperm. This study identifies progesterone (P) as the primary steroid responsible for mediating sperm chemoattraction in humans.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prolactin decreases LPS-induced inflammatory cytokines by inhibiting TLR-4/NFκB signaling in the human placenta.

Molecular human reproduction·2019
Same author

Prolactin selectively inhibits the LPS-induced chemokine secretion of human foetal membranes.

The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·2019
Same author

[Pilot tests using molecular diagnostic assay cervicovaginal infection during pregnancy].

Ginecologia y obstetricia de Mexico·2018
Same author

Progesterone suppresses the lipopolysaccharide-induced pro-inflammatory response in primary mononuclear cells isolated from human placental blood.

Immunological investigations·2017
Same author

Control of overweight and obesity in childhood through education in meal time habits. The 'good manners for a healthy future' programme.

Pediatric obesity·2015
Same author

In vitro progesterone modulation on bacterial endotoxin-induced production of IL-1β, TNFα, IL-6, IL-8, IL-10, MIP-1α, and MMP-9 in pre-labor human term placenta.

Reproductive biology and endocrinology : RB&E·2015

Area of Science:

  • Reproductive Biology
  • Endocrinology
  • Sperm Physiology

Background:

  • Human follicular fluid (FF) plays a crucial role in reproductive processes.
  • Identifying chemoattractants for sperm is essential for understanding fertilization.
  • The molecular basis of sperm chemotaxis in humans remains incompletely understood.

Purpose of the Study:

  • To elucidate the molecular identity of the sperm chemotactic signal present in human FF.
  • To characterize the physicochemical properties of sperm chemoattractants in FF.

Main Methods:

  • Fractionation of human FF to isolate active components.
  • Physicochemical characterization of isolated compounds.
  • Assay of chemotactic activity using a double-chamber device.

Related Experiment Videos

  • Dose-response analysis of individual steroids for sperm chemotactic properties.

    • Main Results:

    • Sperm chemotaxis was linked to a lipid-like molecule within FF.
    • Progesterone (P) was the only steroid demonstrating significant sperm chemotactic activity.
    • Chemotactic activity was dose-dependent for P.

    Conclusions:

    • Progesterone (P) is identified as a key mediator of sperm chemoattraction in humans.
    • P, the predominant steroid in FF, acts as a chemoattractant for sperm.
    • This finding provides critical insights into the mechanisms of human fertilization.