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Manipulation of sperm before intracytoplasmic sperm injection improves fertilization rates
1Human Reproduction Unit, Royal North Shore Hospital, St. Leonards, New South Wales, Australia.
Objective:
To determine the effect of sperm manipulation before intracytoplasmic sperm injection (ICSI) on fertilization rates.
Design:
Three methods of sperm manipulation before direct ICSI were compared in two sibling oocyte trials.
Setting:
In vitro fertilization unit within a teaching hospital.
Patients:
Patients undergoing infertility treatment using ICSI.
Interventions:
Oocytes were inseminated by ICSI.
Main Outcome Measure:
Fertilization rate.
Results:
In the first trial, a standard manipulation technique gave a 67% fertilization rate compared with 64% with a minimal manipulation technique. In the second trial the standard technique gave a significantly greater fertilization rate compared with no prior manipulation (67% versus 45%).
Conclusions:
Manipulation of sperm before direct ICSI is not mandatory for fertilization but significantly improves the fertilization rate.