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Immunological tolerance to xenogeneic grafts induced by cyclophosphamide in adult animals
Chernyakhovskaya IYu1, L N Fontalin
1N.F. Gamaleya Research Institute for Epidemiology and Microbiology, Russian Academy of Medical Sciences, Moscow, Russia.
Data on tolerance of mice to rat tissue grafts obtained during the last 20 years are presented in this article. The method of tolerance induction, which spared recipients' lymphoid tissue and which needed no supporting antigen or immunodepressant injection, is described. These tolerant mice had long-lasting (more than 6 months) xenograft survival, had no cytotoxic antibodies and delayed-type hypersensitivity (DTH) reaction to donor antigens but showed only a partial decrease in the levels of mixed lymphocyte reaction (MLR) and non-cytotoxic antibody production. According to indirect evidence, tolerance arose as a result of clonal deletion and was maintained subsequently by antigen-specific T-suppressor cells and Thy-negative lymphocytes.
Data on tolerance of mice to rat tissue grafts obtained during the last 20 years are presented in this article. The method of tolerance induction, which spared recipients' lymphoid tissue and which needed no supporting antigen or immunodepressant injection, is described. These tolerant mice had long-lasting (more than 6 months) xenograft survival, had no cytotoxic antibodies and delayed-type hypersensitivity (DTH) reaction to donor antigens but showed only a partial decrease in the levels of mixed lymphocyte reaction (MLR) and non-cytotoxic antibody production. According to indirect evidence, tolerance arose as a result of clonal deletion and was maintained subsequently by antigen-specific T-suppressor cells and Thy-negative lymphocytes.