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Updated: Aug 14, 2026

Functional Cloning Using a Xenopus Oocyte Expression System
Published on: January 30, 2016
From clones of cells to clones of genes
1Basel Institute for Immunology, Switzerland.
Abstract:
Three approaches to study the immune system are presented. First is the limiting dilution analysis, second is the analysis of the polypeptide patterns of lymphocytes by two-dimensional gel electrophoresis, and the third is the utilization of a partitioned cDNA library for establishing a gene catalog and proteinpaedia. All the approaches were used to study clonal diversity and clonal heterogeneity. The gene catalog which is already established is available to recover genes expressed in various lymphocyte clones.
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