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Isolation of brefeldin A
1Department of Veterinary Physiology and Pharmacology, Texas A&M University, College Station 77843, USA.
General Pharmacology
|March 1, 1995
Abstract:
1. A chromatographic method for the isolation of brefeldin A (BFA) from the culture medium of Penicillium brefeldianis has been developed. BFA is adsorbed on a reversed-phase (C18) silica, desorbed by methanol, and the crystalline BFA precipitated from the concentrated aqueous solution. The yield of crystalline BFA is 121 mg/l of culture medium from a sample containing 204 mg/l determined by analytical HPLC. 2. The detection limit of BFA is 50 ng by analytical HPLC and BFA in the mother liquor of crystallization is recovered by preparative HPLC.