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Age-related changes in [3H]baclofen binding in mouse cerebellum
1Department of Pharmacology, College of Pharmacy, Nihon University, Chiba, Japan.
Abstract:
1. Specific [3H]baclofen binding to cerebellar membranes from 1-month-old (young), 8-month-old (older) and 20-month-old (aged) mice was lower than that to membranes from 3-month-old mice (mature adult), whereas in cerebral cortical membranes there were no age-related changes in [3H]baclofen binding among the four age groups. 2. Scatchard analysis revealed that the density of cerebellar GABAB receptors significantly decreased during aging. 3. There were no age-related changes in the inhibitory effect of Gpp(NH)p on [3H]baclofen binding. 4. These results suggest that the characteristics of GABAB receptors in the cerebellum change during aging without any alteration in the coupling of the receptor to guanine nucleotide regulatory proteins.