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Rapid identification of Streptococcus pneumoniae by PCR amplification of ribosomal DNA spacer region
K Saruta1, T Matsunaga, S Hoshina
1Department of Laboratory Medicine, Jikei University School of Medicine, Tokyo, Japan.
FEMS Microbiology Letters
|October 1, 1995
Abstract:
Streptococcus pneumoniae is one of the important human pathogens in clinical microbiology. A polymerase chain reaction assay was designed to detect and identify S. pneumoniae through amplification of the ribosomal DNA spacer regions between the pneumococcal 16S-23S ribosomal RNA genes. Thirty-two Streptococcus and non-Streptococcus strains were tested to verify the specificity of the assay, and only S. pneumoniae strains gave a positive reaction. This method is a powerful technique for the rapid identification of S. pneumoniae.