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Human TAFII250 interacts with RAP74: implications for RNA polymerase II initiation
1Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720-3204, USA.
Genes & Development
|November 15, 1995
Summary
The TAFII250 protein interacts with RAP74, a subunit of transcription factor IIF. This interaction is vital for cell viability and RNA polymerase II transcription initiation.
Area of Science:
- Molecular Biology
- Gene Transcription
- Protein-protein Interactions
Background:
- Accurate transcription by RNA polymerase II depends on complex assembly.
- TFIID, containing TBP and TAFs, binds DNA and is crucial for initiation.
- The specific roles of TAFs in transcription initiation and their interactions with other factors are not fully understood.
Purpose of the Study:
- To investigate the interaction between TAFII250 and RAP74.
- To elucidate the functional significance of this interaction in transcription and cell viability.
Main Methods:
- In vitro binding assays to map interaction interfaces.
- In vivo complementation of a temperature-sensitive TAFII250 cell line.
Main Results:
- Specific interaction identified between TAFII250 and RAP74.
- Recognition interfaces between TAFII250 and RAP74 were mapped.
- The interaction between TAFII250 and RAP74 is essential for cell viability.
Conclusions:
- TAFII250 selectively interacts with RAP74, a subunit of transcription factor IIF.
- This interaction is critical for RNA polymerase II transcription initiation.
- The TAFII250-RAP74 interaction plays a key role in the assembly of active transcription complexes and cell survival.
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