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Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations
Published on: November 7, 2019
Characterization of a cDNA clone encoding E2-20K, a murine ubiquitin-conjugating enzyme
I Wefes1, P Kaiser, R Schneider
1Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
Gene
|October 3, 1995
Summary
The ubiquitin-conjugating enzyme E2-20K is crucial for erythroid differentiation. This study reports the murine cDNA sequence for E2-20K, revealing its presence in various tissues.
Area of Science:
- Molecular Biology
- Cellular Differentiation
- Gene Expression
Background:
- The 20-kDa ubiquitin-conjugating enzyme, E2-20K, plays a role in cellular processes.
- E2-20K expression is specifically upregulated during late-stage erythroid differentiation.
Purpose of the Study:
- To determine the full-length sequence of the murine cDNA encoding E2-20K.
- To investigate the expression pattern of E2-20K transcripts in different tissues.
Main Methods:
- cDNA sequencing of murine E2-20K.
- Northern blot analysis to detect E2-20K mRNA transcripts.
Main Results:
- The complete nucleotide sequence of the murine E2-20K cDNA was determined.
- Northern blot analysis revealed two major polyadenylated transcripts of 3.5 kb and 6.5 kb.
- These transcripts were found at comparable levels in various nonerythroid tissues, suggesting a broader role beyond erythropoiesis.
Conclusions:
- The murine E2-20K cDNA sequence provides a basis for further functional studies.
- E2-20K is expressed in multiple tissues, indicating potential functions in diverse cellular contexts.
- The differential induction during erythroid differentiation suggests a specific regulatory mechanism for this cell lineage.
