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pDblet, a stable autonomously replicating shuttle vector for Schizosaccharomyces pombe

C Brun1, D D Dubey, J A Huberman

  • 1Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.

Gene
|October 16, 1995
PubMed
Summary

A new plasmid, pDblet, offers a stable shuttle vector for fission yeast (Schizosaccharomyces pombe). It features high transformation efficiency and mitotic stability for molecular biology applications.

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Area of Science:

  • Molecular Biology
  • Yeast Genetics

Background:

  • Existing Schizosaccharomyces pombe (Sp) vectors present limitations for molecular biology applications.
  • Development of novel vectors is crucial for advancing research in fission yeast.

Purpose of the Study:

  • To construct a new multipurpose, stable shuttle vector for Schizosaccharomyces pombe (Sp).
  • To design a vector overcoming limitations of previous Sp vectors, enhancing ease of use and stability.

Main Methods:

  • Construction of the pDblet plasmid.
  • Incorporation of the Sp ura4 gene as a selectable marker.
  • Inclusion of a novel, highly efficient autonomously replicating sequence (ARS) element.

Main Results:

  • The pDblet vector demonstrates high transformation efficiency in Sp.

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  • It exhibits high mitotic stability and maintains a low copy number.
  • The ARS element ensures stable monomer maintenance within Sp cells.

    • Conclusions:

    • The pDblet vector is a valuable tool for fission yeast molecular biology.
    • Its stability, efficiency, and ease of use make it superior to previous Sp vectors.