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Sequence of a gene encoding periplasmic Pseudomonas syringae ankyrin
1Program in Molecular Biology, Utah State University, Logan 84322-5305, USA.
Gene
|October 16, 1995
Summary
Researchers isolated an ankyrin (Ank) gene from the plant pathogen Pseudomonas syringae. This gene encodes a protein with similarities to ankyrin domains found in mammals, bacteria, animals, and plants.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Ankyrin (Ank) proteins are crucial structural components in various organisms.
- Understanding the diversity of ankyrin genes provides insights into cellular functions across species.
Purpose of the Study:
- To isolate and characterize the ankyrin gene from Pseudomonas syringae pathovar syringae strain 61 (Pss61).
- To investigate the evolutionary conservation of ankyrin domains.
Main Methods:
- Isolation of the ankyrin gene from a Pss61 genomic library.
- Bioinformatic analysis to determine the encoded polypeptide's characteristics and homology.
Main Results:
- Successfully isolated the ankyrin gene from Pss61.
- The gene encodes an 183-amino-acid polypeptide.
- This polypeptide exhibits homology to the 33-amino-acid repeat domain of mammalian ankyrin and its homologs in other species.
Conclusions:
- The ankyrin gene is present in the plant pathogen Pseudomonas syringae.
- The identified ankyrin polypeptide shares conserved structural features with ankyrins across diverse taxa, suggesting a conserved functional role.